Just Passing Through: FALCON(R) HTS FluoroBlok Inserts

Becton Dickinson's Falcon® HTS FluoroBlock Inserts

So you're studying the movement of cells toward a chemical gradient. You'll do multiple time-consuming manipulations--cell scraping, transferring membrane inserts, manual fixation, staining, and microscope counting. Or will you? With a new "radiation opaque" membrane insert for real-time fluorescence studies, you could get your information directly in a matter of minutes.

FluoroBlok is a proprietary colored PET membrane insert that fits into an individual well of a 24-well microplate. The membrane is available in three pore sizes (1, 3, and 8 µm). Besides cell migration and chemotaxis, applications include tumor cell/bacterial invasion, cell-cell adhesion, signaling, and leakage to assess tightness of cell junctions. Cell monolayer (e.g., Caco-2 cell) permeability can be assayed as a model system for an oral bioavailability assay. Since there is a linear relationship between fluorescence and cell number, the number of migrating cells can be quantified. Cell-based high throughput...