Out of the Blue
GenomicsOne's TrueBlue Cloning Scheme For the past two decades, cloners have relied on lacZ*-based color selection vectors to identify cloned inserts. These systems are based on the insertional inactivation of the lacZ* gene fragment and are (deceptively) simple. The lacZ gene encodes the ß-galactosidase (ßGal) protein, which acts on an indicator substrate to produce blue colonies. Thus, in a cloning experiment, white colonies indicate that the DNA of interest has been inserted into t
Jul 9, 2000
![]() GenomicsOne's TrueBlue Cloning Scheme |
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