Pain-free Cloning?

Imagine cloning a gene into several different expression vectors without restriction enzymes or ligations. Invitrogen's Echo Cloning System promises just that. Based on the Univector Plasmid-fusion System developed by Steve Elledge at Baylor College of Medicine, the Echo System avoids having to PCR amplify and sequence an insert every time it is subcloned into a new vector.1 The newly released Echo System combines Invitrogen's proven TOPO® Cloning System for five-minute cloning of PCR produ

Amy Francis
Jan 23, 2000

Imagine cloning a gene into several different expression vectors without restriction enzymes or ligations. Invitrogen's Echo Cloning System promises just that. Based on the Univector Plasmid-fusion System developed by Steve Elledge at Baylor College of Medicine, the Echo System avoids having to PCR amplify and sequence an insert every time it is subcloned into a new vector.1 The newly released Echo System combines Invitrogen's proven TOPO® Cloning System for five-minute cloning of PCR products with the Univector System to rapidly transfer clones into several different protein expression vectors.


Invitrogen's Echo Cloning System
The Echo System is composed of a donor and acceptor vector that are recombined into a single protein expression vector by Cre-lox-mediated site-specific recombination. LoxP and loxH elements are 34 bp sequences that are recognized by the 35 kDa Cre recombinase. Binding by Cre recombinase brings together the lox sequences on the...

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