Researchers interested in evaluating the interaction of transcription factors with their target sequences commonly employ the electrophoretic mobility shift assay, more commonly known as the "gel-shift." Gel-shift assays can provide crucial information on the binding of transcription factors to their consensus sequences, but because the technique relies on the use of radiolabeled nucleotides, it can be cumbersome and time-consuming. A single gel-shift assay can take days to complete, and in the absence of immunological evidence (i.e., using antibodies for specific transcription factors to "supershift" a complex), results can be hard to interpret. Imagine being able to obtain the same data in a fraction of the time, with both increased specificity and sensitivity. Thanks to the Trans-AM™ Transcription Factor Assay kit, from Active Motif of Carlsbad, Calif., this dream has now become a reality-if you're investigating the right transcription factors.
|Courtesy Active Motif|
Active Motif's Trans-AM Assay System
Hervé Le Calvez, Trans-AM product specialist at Active Motif, explains that the Trans-AM NF-kB assay can be completed in less than four hours, whereas a similar experiment with gel-shift can take up to seven days. In addition to convenience, the Trans-AM technology offers approximately ten-fold more sensitivity than a gel-shift assay, making Trans-AM an attractive alternative to gel-shifts. Currently, the Trans-AM technology is available for the detection of both the p50 and p65 members of the NF-kB family. In the future, expect to see Trans-AM kits for other transcription factors, including AP-1 and CREB.
--John Piper (email@example.com)
For More Information
(877) 222-9543, www.activemotif.com