ADVERTISEMENT
ADVERTISEMENT

Paradigm Gel Shift

Researchers interested in evaluating the interaction of transcription factors with their target sequences commonly employ the electrophoretic mobility shift assay, more commonly known as the "gel-shift." Gel-shift assays can provide crucial information on the binding of transcription factors to their consensus sequences, but because the technique relies on the use of radiolabeled nucleotides, it can be cumbersome and time-consuming. A single gel-shift assay can take days to complete, and in the

John Piper

Researchers interested in evaluating the interaction of transcription factors with their target sequences commonly employ the electrophoretic mobility shift assay, more commonly known as the "gel-shift." Gel-shift assays can provide crucial information on the binding of transcription factors to their consensus sequences, but because the technique relies on the use of radiolabeled nucleotides, it can be cumbersome and time-consuming. A single gel-shift assay can take days to complete, and in the absence of immunological evidence (i.e., using antibodies for specific transcription factors to "supershift" a complex), results can be hard to interpret. Imagine being able to obtain the same data in a fraction of the time, with both increased specificity and sensitivity. Thanks to the Trans-AM Transcription Factor Assay kit, from Active Motif of Carlsbad, Calif., this dream has now become a reality-if you're investigating the right transcription factors.

Courtesy Active Motif

Active Motif's Trans-AM Assay System
The flagship...

Interested in reading more?

Become a Member of

Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?
ADVERTISEMENT