Real Time PCR

Quantitative PCR with no gel? Impossible! Now with the ABI PRISM 7700 Sequence Detection System, real time PCR has become a reality. Combining a novel chemistry with a fully integrated PCR amplification and detection system, the 7700 System is a unique, closed-tube system that allows for real time quantitative PCR without sample processing or electrophoresis. At the core of the system is a 5' nuclease assay conducted with the TaqMan¨ PCR Reagent Kit utilizing a fluorogenic probe that has b

The Scientist Staff
Nov 23, 1997

Quantitative PCR with no gel? Impossible! Now with the ABI PRISM 7700 Sequence Detection System, real time PCR has become a reality. Combining a novel chemistry with a fully integrated PCR amplification and detection system, the 7700 System is a unique, closed-tube system that allows for real time quantitative PCR without sample processing or electrophoresis.

At the core of the system is a 5' nuclease assay conducted with the TaqMan¨ PCR Reagent Kit utilizing a fluorogenic probe that has both a reporter and quencher dye attached. When both dyes are attached to the probe, reporter dye emission is quenched. However, once annealed to the target sequence and put into a PCR reaction, the nuclease activity of the Taq polymerase cleaves the reporter dye from the probe, resulting in a fluorescent signal. The fluorescent signal is monitored at every cycle as additional reporter dye molecules accumulate.

Real time PCR offers numerous...

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