Promega Imagine a world without 35S methionine. It's easy with Promega's TranscendTM Non-Radioactive Translation Detection Systems. Utilizing Promega's novel biotinylated lysine tRNA, these systems can be used to detect in vitro translation products after blotting with a variety of detectors, including Promega's own TranscenTM chemiluminescent substrates. No more wipe tests, no more radioactive waste disposal headaches. And results can be obtained in half the time.
![]() One microliter of TNR translation reaction was spearated on a SDS-PAGE gel, blotted onto a PVDF membrane. The biotinylated proteins were detected with Streptavid-HRP and TranscendTM Chemiluminescent Substrates. (Lane 1 beta-galactosidase, Lanes 2 CAT and Lane 3 luciferase) |
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