Scale It Up

Eppendorf-5 Prime's PERFECTprep-96VAC Quad Vacuum Manifold High throughput may be the wave of the future, but most laboratories must still perform smaller-scale procedures at various steps in an investigation. For the many researchers who don't have access to automated systems for DNA purification, Eppendorf-5 Prime Inc. of Boulder, Colo., offers the PERFECTprep-96VAC Quad Vacuum Manifold, a tool for manually purifying DNA rapidly. The system is based on a time-tested alkaline lysis protocol

Aileen Constans
Jun 25, 2000


Eppendorf-5 Prime's PERFECTprep-96VAC Quad Vacuum Manifold
High throughput may be the wave of the future, but most laboratories must still perform smaller-scale procedures at various steps in an investigation. For the many researchers who don't have access to automated systems for DNA purification, Eppendorf-5 Prime Inc. of Boulder, Colo., offers the PERFECTprep-96VAC Quad Vacuum Manifold, a tool for manually purifying DNA rapidly.

The system is based on a time-tested alkaline lysis protocol and uses vacuum filtration to isolate high-purity DNA for automated fluorescent sequencing. The manifold processes from one to four 96-well plates, offering users the ability to purify up to 384 samples simultaneously in one and a half hours. According to sales manager Clay Huber, the Quad Vacuum Manifolds have become so popular that the company "can't make enough of them."

The technology behind the PERFECTprep system is not new. The system itself was developed in the early 1990s and has been revamped over the years for use in automated platforms. It now provides the well-to-well, plate-to-plate, and run-to-run consistency needed in high-throughput sequencing facilities. Huber estimates that the average cost per prep is about 50 cents.

--Aileen Constans (aconstans@the-scientist.com)

For More Information
Eppendorf 5 Prime
(800) 533-5703
www.highthroughput.com