SearchLight Increases Throughput

ELISAs, a quick and reliable way of quantifying protein concentrations, have a significant drawback in the age of high-throughput experiments: Each ELISA only measures the concentration of a single protein. Although the amount of sample volume required to test for a single analyte is not large, volume can become a limiting factor when assaying several proteins. The Pierce Boston Technology Center offers a way around this problem with its new SearchLight™ Proteome Arrays, which measure the

Amy Adams
Oct 14, 2001
ELISAs, a quick and reliable way of quantifying protein concentrations, have a significant drawback in the age of high-throughput experiments: Each ELISA only measures the concentration of a single protein. Although the amount of sample volume required to test for a single analyte is not large, volume can become a limiting factor when assaying several proteins. The Pierce Boston Technology Center offers a way around this problem with its new SearchLight™ Proteome Arrays, which measure the concentration of up to nine proteins at a time.

Each well of a 96-well SearchLight array is spotted with two to nine target-specific antibodies. For instance, the company's human Th1/Th2 cytokine array detects interleukins (IL)-2, 4, 5, 8, 10, 12p70, and 13, plus IFN-* and TNF-*. With 14 wells taken up by standards, researchers have room for 41 different samples in duplicate, or up to 369 experiments per plate. The company can also make...

Interested in reading more?

Become a Member of

Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?