ELISAs, a quick and reliable way of quantifying protein concentrations, have a significant drawback in the age of high-throughput experiments: Each ELISA only measures the concentration of a single protein. Although the amount of sample volume required to test for a single analyte is not large, volume can become a limiting factor when assaying several proteins. The Pierce Boston Technology Center offers a way around this problem with its new SearchLight™ Proteome Arrays, which measure the concentration of up to nine proteins at a time.
Each well of a 96-well SearchLight array is spotted with two to nine target-specific antibodies. For instance, the company's human Th1/Th2 cytokine array detects interleukins (IL)-2, 4, 5, 8, 10, 12p70, and 13, plus IFN-* and TNF-*. With 14 wells taken up by standards, researchers have room for 41 different samples in duplicate, or up to 369 experiments per plate. The company can also make...
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