Luminex's unique microsphere addressing system
Luminex uses a proprietary dyeing process to label polystyrene microspheres with precise ratios of two spectrally distinguishable fluorophores. This process generates 100 different microspheres, each with a unique fluorescent signature. Assay reagents such as antibodies are then linked to "addressed" microsphere sets, and specific reactants from biological samples (antigens) detected with reporter molecules conjugated to a third fluorophore. After assaying, the microsphere suspension (Suspension Array™) is sorted and interrogated individually in a fluid system that passes through two separated laser beams: red to classify the microspheres by their addresses...
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