Grow 'n' Glow GFP Two Hybrid System
Developed by scientists at the Max Planck Institute, the Grow 'n' Glow system uses three vectors: pGNG1, the GFP reporter plasmid; pEG202, the bait plasmid; and pJG4-5, the prey plasmid. Screening a known protein for contacts with a library of unknown proteins first requires cloning the known protein into the bait plasmid to produce an in-frame fusion between the known protein coding sequence and pEG202's LexA DNA-binding domain. This is the...
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