Totally Tubular PCR

Almost everything you need for PCR with MBP's EasyStartTM Tubes Comparison of PCR reactions uing MBP's EasyStartTM Tubes and control reactions made from frozen stock solutions. Each 50 micoliter reaction contained 200 ng mouse genomic DNA and primers for the single copy c-fos gene, which produces a 707 base pair product. The reaction pairs wsere run on agarose gels with a molecular weight marker. The reactions on th left were run with Amplitaq Gold (Perkin Elmer) and on the right with Boehring

The Scientist Staff
Jul 20, 1997

Almost everything you need for PCR with MBP's EasyStartTM Tubes


Comparison of PCR reactions uing MBP's EasyStartTM Tubes and control reactions made from frozen stock solutions. Each 50 micoliter reaction contained 200 ng mouse genomic DNA and primers for the single copy c-fos gene, which produces a 707 base pair product. The reaction pairs wsere run on agarose gels with a molecular weight marker. The reactions on th left were run with Amplitaq Gold (Perkin Elmer) and on the right with Boehringer Mannheim's Taq Polymerase.
Your PCR reaction didn't work. Even the controls didn't work. Your colleagues just shake their heads and say to do it again. This is a familiar scenario in molecular biology labs everywhere. In the last year, several products have appeared in the market to take some of the guesswork out of PCR. Starting with this issue, LabConsumer will be profiling some of these...

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