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Western Blots Go High-Throughput

To drive the proteomics revolution forward, scientists must develop high-throughput research tools. One of these tools is the new Multi-Replica Blotting Kit by Rockville, Md.-based 20/20 Gene Systems Inc. Also called the Layered Proteomics Analysis System for 1-D Gels, the kit allows investigators to make 10 replicate blots from a single one-dimensional protein gel. According to company spokesperson, Liz Marcus, the kit allows researchers to study protein gels faster and more efficiently than co

Ll Lane
To drive the proteomics revolution forward, scientists must develop high-throughput research tools. One of these tools is the new Multi-Replica Blotting Kit by Rockville, Md.-based 20/20 Gene Systems Inc. Also called the Layered Proteomics Analysis System for 1-D Gels, the kit allows investigators to make 10 replicate blots from a single one-dimensional protein gel. According to company spokesperson, Liz Marcus, the kit allows researchers to study protein gels faster and more efficiently than conventional Western blots.

With conventional Western protocols, researchers can prepare one membrane per gel; duplicate filters can be prepared only by running duplicate gels. This approach can lead to blot-to-blot variability. In contrast, with the Multi-Replica Blotting Kit's proprietary transfer buffer and special blotting membranes, scientists can simultaneously make 10 blots from the same sample, reducing sample-to-sample variability. "There's a lot of confidence in being able to work with the same samples," observes Marcus.

The kit...

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