EDITOR'S CHOICE IN Microbiology
I. Turgeman-Grott et al., “Pervasive acquisition of CRISPR memory driven by inter-species mating of archaea can limit gene transfer and influence speciation,” Nat Microbiol, 4:177–86, 2019.
Naturally occurring CRISPR-Cas systems in bacteria and archaea carry DNA memories of invasions by viruses or plasmids. These DNA sequences, called spacers, instruct Cas proteins to destroy the intruders should they enter the cell again. Curiously, several species of halophilic, or salt-loving, archaea isolated from water near Israel’s Mediterranean coast possess spacers matching the DNA of closely related species, report Tel Aviv University’s Uri Gophna and colleagues.
Archaea can mate by latching together with cytoplasmic bridges and exposing their genomes to each other to be recombined. To test whether archaea pick up spacers...
The study shows that “CRISPR can target not only phages and plasmids, but also chromosomal DNA from other species,” says Luciano Marraffini, a microbiologist at Rockefeller University who was not involved with the work. He thinks that in addition to warding off attacks, CRISPR influences evolution by interfering with mating between archaeal species.
Gophna’s team observed another oddity—that in the genetic scramble of interspecies mating, archaea sometimes acquired spacers that instruct their CRISPR systems to pull out chunks of their own DNA, encoding mostly nonessential functions. Gophna sees this “semi-random process” as a way that archaea purge their genome of possibly unhelpful DNA integrated long ago.