Metazoans in the DNAi Club

A chance discovery results in the first report of DNA-based gene silencing in an animal.

Amanda B. Keener
Jul 1, 2015

DNA INTERFERER: Just a few millimeters long, this marine tunicate, Oikopleura dioica, has provided the first glimpse into DNA interference by an animal. HIROKI NISHIDA

EDITOR'S CHOICE IN GENETICS & GENOMICS

The Paper
T. Omotezako et al., “DNA interference: DNA-induced gene silencing in the appendicularian Oikopleura dioica,” Proc R Soc B, 282:20150435, 2015.

Accidental discovery
Scientists often exploit the natural phenomenon of RNA interference (RNAi) to knock down specific genes in model organisms. Although much less common than RNAi, DNAi has been described in plants, ciliates, bacteria, and archaea. And now, thanks to an accidental finding by Tatsuya Omotezako of Osaka University, it appears that DNAi can also silence genes in a metazoan, specifically, the tiny tunicate Oikopleura dioica. “I introduced DNA fragments for another purpose,” Omotezako explained in an e-mail, but instead he found a surprising phenotype—one he would have expected from RNAi.  

As...