The success of downstream applications, such as next generation sequencing (NGS) or western blotting, is often affected by sample quantity and purity. To assess these parameters, researchers can take sample absorbance or fluorescence measurements. However, due to details such as their sensitivity, speed, and sample volume requirements, it is challenging to determine when to employ each method.
Download this ebook from DeNovix to learn about
- The basics of absorbance and fluorescence measurements
- The advantages of measuring sample absorbance, fluorescence, or both
- How to use absorbance ratios to assess sample purity
- How to minimize sample usage for quality control assessments