biochemistry

A Primer on Quantitative Western Blots

Optimizing measurement of relative protein expression changes

Assessing the relative change in protein expression is a valuable and versatile tool for a wide range of basic and applied research. Scientists routinely use quantitative western blots to investigate protein-protein interactions, compare relative levels of proteins across samples, assess relative changes in target abundance, study signal transduction pathways, and measure protein expression changes under different conditions. However, quantitative western blots require additional steps that may introduce workflow bottlenecks or data variability if not performed correctly.

Download this guide from LI-COR to learn the basics of measuring relative protein expression changes using quantitative western blots.

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