One-step RT-qPCR–based applications are increasingly being used in life science research to quickly quantify genes of interest directly from many RNA samples simultaneously. The applications are used in particular and for virus/pathogen detection and for high-throughput screening. Reliance One-Step Multiplex Supermix offers robust, sensitive amplification and detection of up to five targets under a wide range of conditions, including challenging templates, difficult targets, or targets that are in the presence of PCR inhibitors. With its room temperature stability, the supermix can be easily integrated into automated workflows.
"The Reliance One-Step Multiplex Supermix was specifically formulated to offer increased sensitivity and rapid results to address a wide range of RNA detection questions," said Yann Jouvenot, Bio-Rad Marketing Manager, Gene Expression. "Used as part of a workflow along with our CFX Automation System II and PrimePCR Probe Assays, the supermix helps researchers maximize their throughput as well as the quality of their RT-qPCR data," he added.
About Reliance One-Step Multiplex Supermix
Reliance One-Step Multiplex Supermix is a ready-to-use, single-tube reagent mix optimized for sensitive amplification of up to five targets in a single reaction. It is formulated with an optimal enzyme blend including a new Reliance reverse transcriptase and Sso7d fusion polymerase and an advanced buffer to maximize sensitivity and efficiency in multiplex RT-qPCR reactions. Its extended assembled reaction stability (up to 24 hours) is adapted for high-throughput automated workflows. When used as part of a workflow with Bio-Rad's CFX Automation System II and PrimePCR Probe Assays, the Reliance One-Step Multiplex Supermix enables researchers to maximize throughput and quality of RT-qPCR data, processing 40 plates or more in 24 hours.