Chemyx: Enzyme Kinetics Analysis - Tips and Tools

Real-time measurement of the initial stage of an enzyme–substrate reaction is essential for reproducible enzyme kinetics data. Automated direct-flow injection systems couple with various detection and analytics platforms to offer the speed, precision, and flexibility required to carry out the reaction.

Chemyx Inc.
Feb 25, 2018

D

uring an enzymatic reaction, a product is produced, a substrate is used, and the catalyzing enzyme remains unconsumed. Therefore, to analyze the kinetics of an enzyme, a measure of the rate of change in concentration of the substrate being used up and/or the product being formed is typically determined. Conventional parameters such as the Michaelis-Menten constant (KM) and maximum velocity (Vmax) require the concentration of an enzyme to be static while various concentrations of substrate are examined.1 Spectrophotometric assessment of the change in concentration at the start of the enzyme–substrate reaction is preferred for an accurate representation of the kinetics.1
 
Procedural Tip: Get it Right from the Start
 
The beginning of an enzyme–substrate reaction is critical for the estimation of KM and Vmax due to the quick and linear increase in concentration of the product known to occur.1...

 

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Chemyx: Enzyme Kinetics Analysis - Tips and Tools

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