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Chemyx: In a Fix - The Ins and Outs of Laboratory Animal Perfusion

Animal perfusion is important for a plethora of operations including multiple in vivo imaging modalities and targeted gene/drug delivery. Perfusion takes the right reagents, the right parameters, and a steady hand. Perfusion sounds relatively simple, but the subtleties involved makes reproducible execution challenging in practice.

Chemyx Inc.

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erfusion is a vascular technique used to prepare tissues for immunohistochemistry and in situ hybridization analysis in anesthetized animals. This terminal procedure inserts a hypodermic needle into the left ventricle or aorta to inject fixative solutions throughout the circulatory system and targets organs within seconds. The process of perfusion can be relatively easy to explain, yet the subtleties involved in executing this technique make it a challenge in practice. The following discussion outlines essential considerations for efficient whole-body animal perfusion along with perfusion-based applications.

Driving Perfusion: Push it Real Good
 
Pushing fluids through vascular beds of the circulatory system is a core principle of perfusion. This can be done manually using plastic syringes but requires multiple trials to perfect. Even after years of practicing manual perfusion, maintaining steady flow rates can still be a challenge. It is imperative to avoid air bubbles or to vary the flow velocity...

References

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8. T. Stylianopoulos and R.K. Jain, "Combining Two Strategies to Improve Perfusion and Drug Delivery in Solid Tumors," PNAS 12; 100(46): 18632-37, 2013.

 

Meet the Sponsor: 

This article is brought to you by Chemyx, Inc. Syringe Pumps by Chemyx are used in top-level biomedical, pharmaceutical, chemical, and petrochemical research, offering highly precise, consistent, and reproducible fluidic delivery. Chemyx pump devices orchestrate the performance of different technologies that make modern research into novel materials, drugs, and energy resources possible. www.chemyx.com

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