CoolLED PR Piece

New - Fast, Controllable LED Illumination for Fura-2 Ratiometric Calcium Imaging

CoolLED
Aug 16, 2017

Through partnership with The University of Strathclyde, CoolLED are excited to announce the latest leap forward in LED illumination for microscopy – the pE-340fura

The pE-340fura is a bespoke LED illuminator for Fura-2 imaging which also supports everyday fluorescence microscopy in a compact and affordable package.

Launching in September 2017, the pE-300fura will offer;

The right wavelengths

-          A genuine 340nm LED with more than enough intensity at the sample plane to out-perform traditional approaches.

-          A very powerful LED at 380nm which is suitable for both Fura-2 and everyday dyes such as DAPI and Hoechst.

-          A broad white output from 435nm – 645nm allowing sample healthy cells to be distinguished from dying cells using dyes such as Propidium Iodide (PI)or everyday imaging with fluorophores including CFP GFP/FITC, YFP, Tritc/TxRed/mCherry, Cy5 and similar

-          All of this is delivered via UV optimised optics which allow maximum...

Control and compatibility

Based on the highly successful pE-300 Series the pE-340fura has benefits from many of the same features:

-          Can be mounted directly on a vast range of microscopes, old and new.

-          Can be coupled to the microscope via a UV optimised liquid light guide and optional collimator for applications which are particularly sensitive to noise of any type.

-          Can also be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds. Connectivity includes “per channel”, global and a sequence runner.

More than just a replacement for traditional approaches

Pre-launch testing of the pE-340fura has confirmed some exciting benefits for Fura-2 users:

-          The inherent stability of LED illumination reduces the noise level of the light source to a range below that of the wider experiment, reducing false positives and increasing overall precision

-          Higher signal to noise gives cleaner images and data whilst requiring less Fura-2 dye, reducing toxicity and costs.

Find out more: info@coolled.com

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CoolLED PR Piece

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