GloMelt™ Thermal Shift Protein Stability Assay

A rapid and inexpensive fluorescence method for screening conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives).

Biotium
Apr 16, 2018

A rapid and inexpensive fluorescence method for screening conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives).

GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO® Orange

GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR® Green channel of qPCR instruments

Great for high throughput assays, low reaction volumes and low protein concentrations

GloMelt™ dye tolerates DTT very well, unlike PROTEOSTAT® PTS dye

Can be combined with ROX to get very precise replicates in qPCR systems requiring ROX reference dye

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GloMelt™ Thermal Shift Protein Stability Assay

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