GloMelt™ Thermal Shift Protein Stability Assay
A rapid and inexpensive fluorescence method for screening conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives).
A rapid and inexpensive fluorescence method for screening conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives).
GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO® Orange
GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR® Green channel of qPCR instruments
Great for high throughput assays, low reaction volumes and low protein concentrations
GloMelt™ dye tolerates DTT very well, unlike PROTEOSTAT® PTS dye
Can be combined with ROX to get very precise replicates in qPCR systems requiring ROX reference dye
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