Latest Reproducibility Project Study Fails to Replicate

Researchers did not observe the same link between a bacterium and human colon cancer that prior investigators had reported.

kerry grens
Kerry Grens

Kerry served as The Scientist’s news director until 2021. Before joining The Scientist in 2013, she was a stringer for Reuters Health, the senior health and science reporter at...

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Mar 13, 2018

WIKIMEDIA, PATHOIn the latest installment of the Reproducibility Project: Cancer Biology, an independent team attempted to replicate a study from 2012 that found a link between Fusobacterium nucleatum and human colorectal cancers. As the replicators report today (March 13) in eLife, they did not see an increased prevalence of the bacterium in cancerous tissue as the first group reported.

“The difference in F. nucleatum expression between [colorectal carcinoma] and adjacent normal tissues was thus smaller than the original study, and not detected in most samples,” the authors of the latest experiment write in their report.

The replication study is yet another example of the challenges in trying to reproduce the work of other scientists. The Reproducibility Project, which has repeated experiments from 10 studies so far, has had mixed results as far as ending up with the same observations as the original papers.

“[T]he picture is convincing...

Cynthia Sears, a cancer researcher at Johns Hopkins University School of Medicine who reviewed the replication study, writes in a commentary in eLife that the failure to repeat the results does not invalidate the original findings. For one, she writes, the differences could be due to sequencing variations or different patient samples.

“Given that numerous studies have already shown that there is an association between F. nucleatum and the microbiota of human colon cancer . . . the Replication Study is not a reason to change our view of this association,” she writes. “Rather, it provides a critical opportunity to reflect on our growing, yet incomplete, knowledge regarding fusobacteria and colon cancer.”

See “Want to Boost Reproducibility? Get Another Lab Involved