Reverse Transcriptase with Proofreading Capabilities Created

Using directed evolution, researchers selected a DNA polymerase to copy RNA into DNA.

Jun 27, 2016
Alison F. Takemura

Illustration of DNA polymerase being selected to transcribe RNA into DNA in an emulsion PCR reaction UNIVERSITY OF TEXAS AT AUSTIN, JARED ELLEFSON

RNA polymerases are sloppy. A retrovirus with an RNA genome, which is copied into DNA by a reverse transcriptase, is prone to mutations because the enzyme lacks the 3’ to 5’ proofreading capability of DNA polymerases. Scientists have now created a synthetic reverse transcriptase using directed evolution, they reported in a paper published last week (June 24) in Science.

The team, led by by Jared Ellefson and Andrew Ellington at the University of Texas, Austin, has dubbed this enzyme a reverse transcription “xenopolymerase,” or RTX. In their paper, the authors suggested RTX could be a valuable molecular tool, particularly for transcriptomics.

To amplify transcripts, scientists normally rely on a two-step process: copying RNA into DNA and then amplifying the DNA with a DNA polymerase. But copying RNA into DNA risks mangling the original transcript because reverse transcriptase can’t proofread—a problem exacerbated when using only a few transcripts from a single cell. The xenopolymerase, however, can carry out reverse transcription with significantly greater accuracy. 

“This enzyme could be a game changer for single-cell analyses,” Vitor Pinheiro, who engineers DNA polymerases at University College London and was not involved in the work, told Chemical & Engineering News.

To develop RTX, the team introduced a library of randomly mutated DNA polymerases into E. coli. They then inserted a few RNA bases into DNA sequences required for the polymerase to replicate, challenging the polymerase to transcribe the mixed nucleic acid template. The team added increasing amounts of RNA in subsequent cycles of selection. With just about a dozen amino acid changes and its proofreading ability intact, the DNA polymerase became a reverse transcriptase, as well.

September 2018

The Muscle Issue

The dynamic tissue reveals its secrets


Sponsored Product Updates

StemExpress LeukopakâNow Available in Frozen Format

StemExpress LeukopakâNow Available in Frozen Format

StemExpress, a Folsom, California based leading supplier of human biospecimens, announces the release of frozen Peripheral Blood Leukopaks. Leukopaks provide an enriched source of peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells that can be used in a variety of downstream cell-based applications.

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

Vector Laboratories, a leader in the development and manufacture of labeling and detection reagents for biomedical research, introduces VECTASHIELD® Vibrance™ – antifade mounting media that delivers significant improvements to the immunofluorescence workflow.

Enabling Genomics-Guided Precision Medicine

Enabling Genomics-Guided Precision Medicine

Download this eBook from Qiagen to learn more about the promise of precision medicine and how QCITM Interpret can help deliver better care with better knowledge.

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Download this white paper from Bertin Technologies to learn how to extract and analyze lipid samples from various models!