Menu

Scientists Destroy Entire Chromosome with CRISPR

Multiple DNA breaks at either the centromere or the long arm of the mouse Y chromosome cause it to fragment and disappear.

Aug 1, 2017
Kerry Grens

ISTOCK, POLESNOYResearchers have managed to wipe out an entire mouse chromosome using CRISPR-Cas9. They aimed numerous double-strand breaks at either the centromere or the long arm of the Y chromosome, causing it to fragment and become deleted from XY mouse embryonic stem cells in vitro.

The centromere-targeting approach also resulted in Y-chromosome loss when it was applied to mouse embryos in vivo.

“This study shows that targeted chromosome deletion is achievable and relatively efficient both in vitro and in vivo using CRISPR/Cas genome editing,” the authors, based at the University of Adelaide in Australia, wrote in their report, which appears in the August issue of Molecular Therapy.

The scientists set out to explore whether CRISPR-Cas9 genome editing could be applied to aneuploidies, conditions caused by extra chromosomes. They first cut the centromere of the Y chromosome in 41 places in vitro, and found that in 90 percent of cells the Y chromosome was undetectable, compared to 13 percent in untreated cells. Similarly, cutting the long arm of the Y chromosome in 298 loci resulted in 95 percent of cells losing the chromosome.

The team then treated 27 male mouse zygotes with CRISPR, targeting 41 sites at the Y chromosome centromere. Only eight of the resulting embryos ended up with a Y chromosome.

“This approach should be applicable for other chromosomes and could be utilized in a variety of cellular contexts and species,” the authors write. “Accordingly, we envisage that this strategy will be applied to modeling of aneuploidy syndromes and therapeutic intervention by targeting parental-specific polymorphisms.”

April 2019

Will Car T Cells Smash Tumors?

New trials take the therapy beyond the blood

Marketplace

Sponsored Product Updates

Application of TruBIOME™ to Increase Mouse Model Reproducibility
Application of TruBIOME™ to Increase Mouse Model Reproducibility
With this application note from Taconic, learn about the effects of the microbiome on reproducibility and predictability and how TruBIOME™ helps researchers generate custom microbiota mouse models!
Getting More Consistent Results by Knowing the Quality of Your Protein
Getting More Consistent Results by Knowing the Quality of Your Protein
Download this guide from NanoTemper to learn how to identify and evaluate the quality of your protein samples!
Myth Busting: The Best Way to Use Pure Water in the Lab
Myth Busting: The Best Way to Use Pure Water in the Lab
Download this white paper from ELGA LabWater to learn about the role of pure water in the laboratory and the advantages of in-house water purification!
Shimadzu's New Nexera UHPLC Series with AI and IoT Enhancements Sets Industry Standard for Intelligence, Efficiency and Design
Shimadzu's New Nexera UHPLC Series with AI and IoT Enhancements Sets Industry Standard for Intelligence, Efficiency and Design
Shimadzu Corporation announces the release of the Nexera Ultra High-Performance Liquid Chromatograph series, incorporating artificial intelligence as Analytical Intelligence, allowing systems to detect and resolve issues automatically. The Nexera series makes lab management simple by integrating IoT and device networking, enabling users to easily review instrument status, optimize resource allocation, and achieve higher throughput.