Menu

A comprehensive solution for cell lysis, RNA removal and purification of intact genomic DNA from a wide variety of biological samples, including, but not limited to, cultured cells, blood and mammalian tissues.

May 15, 2019
New England BioLabs

New England Biolabs® (NEB®) today announced the release of its Monarch Genomic DNA Purification Kit. The kit offers extremely effective, high-yield purification of high molecular weight DNA fragments (with peak size >50 kb), making it an excellent choice upstream of long-read sequencing platforms. Furthermore, the inclusion of RNase A, optimized protocols and buffer chemistry of the kit enable researchers to prevent RNA contamination (RNA levels are generally less than 1%) and address a wide variety of sample types without having to purchase any specialized kits.

"We are excited to round out our Monarch product line with one of the most versatile DNA purification kits on the market," says Danielle Freedman, Product Marketing Manager at New England Biolabs. "We anticipate this kit will benefit researchers working with many different sample types, particularly those working with tissues that are notoriously difficult to work with, such as fibrous or fatty tissue."

Because the lysis step of purification has traditionally been the most time consuming, the protocols have been tailored to utilize different buffers depending on sample type, accelerating this step substantially. Moreover, tough-to-lyse samples, such as bacteria and yeast, can be processed with additional steps that enhance lysis. Protocols are also included to enable purification from clinically-relevant samples, such as saliva and cheek swabs. The kit employs a warm elution step, which not only helps to efficiently purify large DNA fragments, but also enables elution of highly-pure DNA in a single elution step.

Other advantages of the kit include:

  • Excellent documentation and detailed protocols
  • Elution can be carried out in as little as 35 µl
  • Elution buffer is formulated for long term storage

Finally, in alignment with NEB's commitment to promoting sustainable practices, the Monarch purification column is manufactured using reduced amounts of plastic, and the kit is packaged using more sustainable, recyclable materials.

The Monarch Genomic DNA Purification Kit complements NEB's qPCR, PCR and NGS workflows, and can be utilized with these product lines accordingly. The columns and buffers are also available separately for added convenience.

June 2019

Living with Bacteria

Can pathogens be converted to commensals?

Marketplace

Sponsored Product Updates

New-Generation Incubator Brings the Latest Technology to Mid-Capacity Microplate Cell Culture
New-Generation Incubator Brings the Latest Technology to Mid-Capacity Microplate Cell Culture
New Thermo Scientific Cytomat 2 C-LiN Series Automated Incubator delivers robust and reliable culture solution for cell-based pharmaceutical applications
Cellecta, Inc. Launches the First Commercially Available Dual-sgRNA Libraries for CRISPRa and CRISPRi Genetic Screens
Cellecta, Inc. Launches the First Commercially Available Dual-sgRNA Libraries for CRISPRa and CRISPRi Genetic Screens
Libraries of pooled lentiviral constructs that express two different sgRNA to all 19,000 human protein-coding genes improve overall gene activation or repression and generate more robust screening results
Novel Antibody-Drug Conjugates: Characterization Tools and Formulation/Process Development
Novel Antibody-Drug Conjugates: Characterization Tools and Formulation/Process Development
In this webinar, learn about antibody-drug conjugates (ADCs) and their function, how to analyze and characterize ADCs, and developing ADC processes and formulations.
Sartorius debuts the Intellicyt iQue3 at CYTO® 2019
Sartorius debuts the Intellicyt iQue3 at CYTO® 2019
Sartorius will be showcasing the latest addition to its cellular analysis portfolio on booth #103 at this year’s CYTO® conference and exhibition, with the launch of the Intellicyt iQue3.