throughput SIN-ning

A new expression cloning method based on SIN replicon particles from the Sindbis alphavirus promises high-throughput mammalian expression cloning.

| 1 min read

Register for free to listen to this article
Listen with Speechify
0:00
1:00
Share

Functional genomics hopes to fill the gap between sequence information and functional information. In the September issue of Nature Biotechnology, Daniel Koller and colleagues at Cytos Biotechnology AG in Zurich, Switzerland, describe the development of an expression cloning system DELphi (discovery of localized proteins) that may help to fill this gap (Nature Biotech 2001, 19:851-855).

They attempted to overcome the limitations of current expression cloning technologies by using Sindbis virus, a single-stranded RNA virus of the Alphavirus genus. The system uses cloned cDNAs and a plasmid encoding the SIN virus-derived replicase. This is co-transfected with a helper plasmid encoding viral structural proteins to generate replication-competent bipartite virus particles, called SIN replicon particles.

Koller et al. show that a single SIN particle was sufficient to initiate an infectious cycle, and infected cells expressing a receptor for a ligand of choice could be selected by flow-cytometric sorting. They validated their DELphi system ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!
Already a member? Login Here

Meet the Author

  • Jonathan Weitzman

    This person does not yet have a bio.
Share
3D illustration of a gold lipid nanoparticle with pink nucleic acid inside of it. Purple and teal spikes stick out from the lipid bilayer representing polyethylene glycol.
February 2025, Issue 1

A Nanoparticle Delivery System for Gene Therapy

A reimagined lipid vehicle for nucleic acids could overcome the limitations of current vectors.

View this Issue
Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

sartorius logo
Considerations for Cell-Based Assays in Immuno-Oncology Research

Considerations for Cell-Based Assays in Immuno-Oncology Research

Lonza
An illustration of animal and tree silhouettes.

From Water Bears to Grizzly Bears: Unusual Animal Models

Taconic Biosciences
Sex Differences in Neurological Research

Sex Differences in Neurological Research

bit.bio logo

Products

Photo of a researcher overseeing large scale production processes in a laboratory.

Scaling Lentiviral Vector Manufacturing for Optimal Productivity

Thermo Fisher Logo
Collage-style urban graphic of wastewater surveillance and treatment

Putting Pathogens to the Test with Wastewater Surveillance

An illustration of an mRNA molecule in front of a multicolored background.

Generating High-Quality mRNA for In Vivo Delivery with lipid nanoparticles

Thermo Fisher Logo
Tecan Logo

Tecan introduces Veya: bringing digital, scalable automation to labs worldwide