Courtesy of Nextgen Sciences
NextGen Sciences of Cambridge, UK,
A bacterial artificial chromosome (BAC) sequence allows the vector to propagate in bacteria. Homologous recombination by cotransfection of
Other systems produce a mixture of recombinant and parental virus and require a lengthy plaque assay to screen for viruses containing the gene of interest, says Littlewood. "With our technology, you only make 100% recombinant virus; there's no parental virus," says coinventor Linda King of Oxford Brookes University, Oxford, UK. "You don't have to separate the parental virus away from the recombinant virus at a later step, and therefore you would reduce all those time-consuming, labor-intensive steps."
NextGen licensed the technology from Oxford Brookes, which continues to sell the kit through Oxford Expression Technologies (