Courtesy of Upstate USA
Researchers often use immunoprecipitation (IP) to isolate specific proteins from complex samples or to identify protein-protein interactions. But the method, in which an antibody bound to protein A or G and tethered to a resin pulls an antigen out of a protein mixture, involves a number of liquid-handling steps that can lower yield, limit reproducibility, and preclude high-throughput processing. Plus, the resin (typically agarose beads) can interfere sterically with downstream assays, as it is difficult to dissociate it from the antigen.
The Catch and Release IP system from Upstate Group