Though recognized as prime drug targets, membrane proteins largely remain
Enter AB Vector
AB Vector offers two baculovirus-based vector lines: the ER series, for G protein-coupled receptors (GPCRs) and other receptors that must be translocated to the ER and often glycosylated for proper folding; and the C series, for cytosolic and cytoplasmic proteins that do not require translocation. The C Series vectors encode for the cytoplasmic chaperones Hsp40 and Hsp70; the ER series vectors encode for protein disulfide isomerase (PDI), which facilitates disulfide bond formation, and calreticulin, a glycoprotein-folding chaperone. All constructs include a GFP marker to monitor virus propagation.
Mendez, who calls membrane proteins the "next frontier" of the protein-expression industry, is in the early stages of evaluating the technology on GPCRs and Trp channels. Mendez says he has seen an increase in expression over conventional baculovirus vectors and believes this approach to modifying the baculovirus vectors will be the key to membrane protein overexpression.
The ProFold system costs $695 for five transfections. It is compatible with baculovirus systems from BD Biosciences-Clontech and BD Biosciences-Pharmingen. AB Vector also offers custom services using the ProFold technology.
- Aileen Constans