Courtesy of Promega
Have you ever needed a red fluorescent version of a protein, but only green was available? Promega
"You do only one cloning to get the fusion protein and then you expose the cell to different [ligands]," explains Covalys spokesperson Tom Gibbs, "whereas you have to reclone to get a different fusion protein for each variant of [green fluorescent protein]."
Currently available ligands enable labeling of fusion proteins in live or fixed mammalian cells or gels and isolating fusion proteins from complex mixtures. "But whenever we talk about this technology, our audience...