Shifting Away from Gel Shifts

While researchers have long relied on electrophoretic mobility-shift assays (called gel shifts) to identify which transcription factors bind to DNA samples, the method can be time-consuming and requires the use of radiolabeled nucleotide to track protein-DNA complexes.Now Novagen http://www.novagen.com, a brand of San Diego-based EMD Biosciences, is offering a new alternative to gel shifts with its NoShift Transcription Factor Assay Kit. The kit eliminates gels altogether, immobilizing DNA insid

Tariq Malik
Jul 18, 2004

While researchers have long relied on electrophoretic mobility-shift assays (called gel shifts) to identify which transcription factors bind to DNA samples, the method can be time-consuming and requires the use of radiolabeled nucleotide to track protein-DNA complexes.

Now Novagen http://www.novagen.com, a brand of San Diego-based EMD Biosciences, is offering a new alternative to gel shifts with its NoShift Transcription Factor Assay Kit. The kit eliminates gels altogether, immobilizing DNA inside 96-well microplates instead, where binding factors can be isolated quickly and without radioactive labels.

Microplate assay users "get their data relatively quick," says Michael Atchison, a University of Pennsylvania biochemistry professor. "Whereas in a gel shift, results can go anywhere from a few minutes to a few days, depending on the film exposure length." The NoShift kit can perform a complete 96-reaction run in about five hours, according to Annette Pownell, supervisor of technical services for Novagen.

NoShift microplates...

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