Courtesy of Waters Corporation
Waters' Quattro Premier XE Mass Spectrometer
Last summer got off to a hot start at the June 2005 American Society for Mass Spectrometry meeting in San Antonio, Texas. With new product releases ranging from labeling reagents for quantitative proteomics to breakthrough hybrid mass spectrometry technology, a variety of companies took advantage of the large gathering of potential customers to introduce their wares. This roundup highlights several new and noteworthy offerings.
BUZZWORTHY MASS SPECTROMETERS
John Yates of The Scripps Research...
Boston-based PerkinElmer Life and Analytical Sciences
The first is a high-throughput sample-preparation method that uses membrane absorbers to capture abundant carrier proteins such as albumin, and a proprietary chemistry (developed by partner Vivascience of Hannover, Germany) to release low-abundance biomarkers that are associated with the carrier proteins directly onto a MALDI plate, leaving the carrier proteins behind.
The platform's other components are PerkinElmer's high-resolution prOTOF mass spectrometer, which can acquire spectra over a broad mass range (700–10,000 daltons) in one acquisition, and new biomarker analysis software developed with partner companies Nonlinear Dynamics and Predictive Diagnostics, to handle the resulting data. Lopez says the company has a paper in press describing the platform's use for the discovery of a diagnostic pattern for Alzheimer disease.
ALTERNATIVE TO ICAT
Another advantage, says Suckau, is that ICPL labeling is done at the level of the intact proteome; the two samples to be analyzed are labeled first, combined, fractionated, and then digested. In ICAT, the proteins are digested before the resulting peptides are labeled, potentially introducing errors because the two samples are digested under different conditions, Suckau says.
Bruker Daltonics, which is comarketing ICPL with Serva, offers software to support quantitative proteomic analysis with the ICPL kits.