linkurl:1. Breakthrough in nano-imaging;, ultra-short X-ray laser pulses may allow researchers to image the structures of a wide range of membrane protein complexes. In contrast to traditional x-ray crystallography, which requires large protein crystals, the new technique can visualize nano-sized crystals with limited radiation damage by combining thousands of diffraction patterns to create 3D structures at sub-nanometer resolution.H.N. Chapman et al., "Femtosecond X-ray protein nanocrystallography,"linkurl: Nature,; 470:73-7, 2011. Evaluated by Thomas Meier, Max Planck Inst Biophysics, Germany; Andrey Kovalevsky and Paul Langan, Los Alamos National Lab; Yuval Mazor and Nathan Nelson, Tel Aviv Univ, Israel; Tetsuji Okada, Gakushuin Univ, Japan; Laurie Betts and Matt Redinbo, Univ North Carolina at Chapel Hill. linkurl:Free F1000 Evaluation; linkurl:2. Identifying the unknown;
Image: flickr, AJC1
Researchers present a novel method for identifying antibody biomarkers for diseases without known antigens. The technique, which involves searching for antibodies that bind...
M.M. Reddy, et al., "Identification of candidate IgG biomarkers for Alzheimer's disease via combinatorial library screening," linkurl:Cell,; 144:132-42, 2011. Evaluated by Angela Vincent, Univ of Oxford, UK; Robert Powers, Univ of Nebraska; Soumitra Ghosh and Kavita Shah, Purdue Univ; Ivan Gerling, Univ Tennessee Health Sci Cen; David Holtzman, Wash Univ School of Med. linkurl:Free F1000 Evaluation; 3. The united states of RNAS.V. Solomatin et al., "Multiple native states reveal persistent ruggedness of an RNA folding landscape," linkurl:Nature,; 463:681-4, 2010. Evaluated by Douglas Turner, Univ Rochester; Daniel MacDougall, Margaret Elvekrog, and Ruben Gonzalez, Columbia Univ; Pascale Legault, Univ de Montreal, Canada; Robert Batey, Univ Colorado. linkurl:Free F1000 Evaluation; linkurl:4. Ribosome assembly up close; A.M. Mulder, et al., "Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit,"linkurl:Science,; 330:673-7, 2010. Evaluations by Junjie Zhang and Michael Levitt, Stanford Univ; Pascale Legault, Univ. Montreal; Mikael Akke, Lund University, Sweden. linkurl:Free F1000 Evaluation; 5. Largest virus imaged on nano-scalecapturedM.M. Seibert et al., "Single mimivirus particles intercepted and imaged with an X-ray laser," linkurl:Nature,; 470:78-81, 2011. Evaluated by Brian Adair and M Amin Arnaout, Harvard Med School; Andrey Kovalevsky and Paul Langan, Los Alamos National Lab; P Shing Ho, Colorado State Univ. linkurl:Free F1000 Evaluation; 6. Recreating virtual membranesS.J. Marrink et al., "The MARTINI force field: coarse grained model for biomolecular simulations," linkurl:J Phys Chem B,; 111:7812-24, 2007. Evaluated Nathan Baker, Pacific Northwest National Lab. linkurl:Free F1000 Evaluation; 7. Studying wet proteinsN.V. Nucci et al., "Site-resolved measurement of water-protein interactions by solution NMR," linkurl:Nat Struct Mol Bio,; 18:245-9, 2011. Evaluated by Yu Chen and Gabriele Varani, Univ of Washington; Gira Bhabha and H Jane Dyson, The Scripps Research Institute. linkurl:Free F1000 Evaluation;

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