A validated high-performance liquid chromatography (HPLC) method still holds one unmonitored variable: water quality. Because water enters the method at every stage—from preparing standards to running the eluent—trace contaminants introduced early propagate through the analysis.
This whitepaper works backward from peak shape to contaminant: split peaks to colloids, shifted polar retention to ionic species, erratic detector response to dissolved gas. It then gives the water grade that keeps each contaminant out, up to Type I at 18.2MΩ.cm.
Download this whitepaper to see
- The minimum acceptable water specifications for manufacturing, cleaning, and analysis
- A direct comparison of baseline stability between bottled and freshly purified water
- Why deionized and distilled water fall short of HPLC requirements
