The Twist TrueAmp Library Preparation Kit delivers consistent, high-quality libraries for NGS target enrichment workflows including challenging low-input, FFPE, and cfDNA samples. Powered by Twist's proprietary TrueAmp polymerase and optimized for enzymatic fragmentation, the workflow achieves high conversion efficiency, low GC bias, and balanced coverage across difficult genomic regions. With 3-hour assay time, under 1.5 hours hands-on, and input flexibility from 1–500 ng, TrueAmp supports accurate variant characterization without sacrificing throughput. Compatible with Illumina platforms, UDIs, and UMIs.
Reliable Uniformity Across Challenging Genomic Regions:
Improved coverage consistency reduces AT- and GC-related dropout, delivering better representation of difficult regions.
High Conversion Efficiency From Low Input and Degraded Samples:
High library yields across low input, FFPE, and variable quality samples help preserve precious material by reducing the need for reruns.
High Fidelity Amplification for Confident Variant Detection:
Twist TrueAmp polymerase minimizes amplification induced errors and bias, supporting accurate detection of low VAF variants.
Product Specifications:
| Specification | Value |
| Assay Time | 3 hours (library preparation only) |
| Hands-On Time | < 1.5 hours |
| Input Quantity | 1-500 ng (50 ng recommended per pool) |
| Sequencing method | Library Prep (Enzymatic Fragmentation) |
| Sample types | Genomic DNA, low-input DNA, FFPE |
| Fragmentation method | Enzymatic |
| Platform compatibility | Illumina |
| Adapter compatibility | UDIs, UMIs |
