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| OXYGEN SUPPLY Medium flow (0.1 ml/min) was provided by a multi-channel peristaltic pump (A) and gas exchange was provided by a coil of thin silicone tubing (B). Channeled elastomer scaffolds (C) 5mm in diameter and 2mm thick were seeded with heart cells at physiologic density (~100 million cells/ml) At left see a scanning electron micrograph of the biorubber scaffold with a parallel channel array and a single channel shown at the beginning and after seven days of cultivation. (Adapted from reference 9.) |
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