Charles Lin, Associate Professor of Dermatology, Harvard University Medical School
Investigating the role of vascular endothelial cell adhesion molecules in the entry of circulating cancer cells into tissue
Immunofluorescent labels may bind to targets other than the specific antigen for which they are designed. In vitro protocols include a washing step to minimize the effect of nonspecific binding, but that's not possible in vivo.
In vivo immunofluorescence protocols differ considerably from in vitro protocols, but that difference allows researchers to add a robust control for nonspecific binding, says Lin.
In vitro immunofluorescence is often performed as a sandwich-type assay,...