Ruedi Aebersold, professor of molecular systems biology, Swiss Federal Institute of Technology, Zurich
Cancer biomarker discovery in serum
If most proteins are glycosylated, most peptides are not. Thus, a whole-cell or whole-serum protein extract can overwhelm a mass spectrometer. Aebersold needed a way to focus only on glycosylated fragments.
Aebersold enriched his samples with a method called solid-phase extraction of N-linked glycopeptides ( Nat Protocols, 2:334-9, 2007). A protein extract is digested to peptides, and treated with sodium periodate to oxidize the sugars to aldehydes....