Selected Genotyping Platforms
|System/Platform||Reaction Method||Pro||Con||Vendor||Relative Cost *|
|Luminex, Bio-Plex||ASPE (see figure), SBE, OLA||• Choice of chemistries for PCR |
• Multiplexing capability (up to 50-plex), especially for multiple variants from the same gene
|• Optimization may be difficult |
• Requires dedicated instrument
|Molecular inversion probes & GeneChip||Probe hybridization||• Ideal for genome-wide studies |
• Can be customized for smaller projects
• Can be outsourced
|• Without outsourcing, requires costly capital investment||Affymetrix||$|
|GoldenGate & BeadArray||ASPE & OLA||Illumina||$|
|Infinium II & BeadArray||ASPE / SBE||Illumina||$|
|TaqMan||FRET (see figure)||• Highly specific and sensitive |
• Simple closed-tube procedure
|• Limited multiplexing |
• Relatively costly
|High-resolution melting-curve analysis||Intercalating dye, hybridization probe (labeled or unlabeled)||• Rapid, inexpensive, suitable for multiplex...|
Interested in reading more?
Become a Member of
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?