User:
Rachel Friedman, a postdoc in the lab of Matthew Krummel, Associate Professor of Pathology at University of California, San Francisco
Project:
Investigating T cell-receptor dynamics during initial response to antigens
Problem:
Friedman images surgically exposed or explanted lymph nodes to examine T cell behavior. The inflammatory response draws nonspecific macrophages and dendritic cells to the lymph node; these emit their own autofluorescent light as bright as the light from the green fluorescent protein (GFP) marker she uses.
Solution:
Friedman compensates for background using dual labels. She injects a recipient mouse with T cells...
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