This webinar will be hosted live and available on-demand
Tuesday, November 22, 2022
2:30 PM Eastern Time
Scientists use in situ hybridization (ISH) to reveal the location of specific nucleic acid sequences within cells, which is a crucial step for understanding gene organization, regulation, and function. In situ hybridization can preserve spatial information, which avoids the masking of cellular heterogeneity that comes with bulk analysis.
In this Technique Talk, Jia Guo will explore the fundamentals of ISH and the development and use of novel in situ hybridization technology to investigate complex biological systems.
- How multiplexed fluorescent ISH works
- Mild yet efficient methods for removing signal
- Ways to amplify signal
- Detecting short RNA species in formalin-fixed paraffin-embedded (FFPE) tissues
Jia Guo, PhD
Biodesign Institute and School of Molecular Sciences
Arizona State University