The release of peptide hormones or neurotransmitters from neuroendocrine cells is essential for the co-ordination of diverse physiological events. Secretory vesicles derived from the trans-Golgi undergo a complex cycle — including maturation, translocation, membrane docking, and fusion of a subpopulation of vesicles with the cell membrane — but many aspects of vesicle dynamics are still unclear. In the March 13
Duncan et al. developed a system to track fluorescently labeled large dense-core secretory vesicles (LDCVs) in living, cultured bovine adrenal chromaffin cells (BCCs). Fluorescent labeling of LDCVs was achieved by transduction of BCCs so as to express the...