Notable

Volume 16 | Issue 13 | 29 | Jun. 24, 2002 Previous | Next New & Notable Selected articles from faculty member reviews | Compiled by Jeffrey M. Perkel The Faculty of 1000 is aWeb-based literature awareness tool published by BioMed Central. For more information visit www.facultyof1000.com. RIBOZYME DESIGN D.H. Burke et al., "Allosteric Hammerhead Ribozyme TRAPs," Biochemistry, 41:6588-94, May 28,

Jeffrey Perkel
Jun 23, 2002
faculty of 1000
Volume 16 | Issue 13 | 29 | Jun. 24, 2002

New & Notable

Selected articles from faculty member reviews | Compiled by Jeffrey M. Perkel


The Faculty of 1000 is a
Web-based literature awareness tool published by BioMed Central. For more information visit www.facultyof1000.com.

RIBOZYME DESIGN
D.H. Burke et al., "Allosteric Hammerhead Ribozyme TRAPs," Biochemistry, 41:6588-94, May 28, 2002.

"Hammerhead ribozyme activity has been upregulated allosterically more than 250-fold with "activator" DNA oligonucleotides that bind not to the ribozyme core, but to an "attenuator" region appended to the ribozyme. The upregulation of single-turnover activity could be increased even further by inclusion of 'antiattenuator' sequence on the activating oligo. Several features of the work offer interesting potential for more general application: (1) The assembly order of the ribozyme, substrate, and activating oligonucleotide affects the final activity level; (2) Neither the activator nor the...