AI models now output thousands of protein designs at once. Because building them individually is cost-prohibitive, researchers rely on pooled assembly. But the PCR step these pools require creates chimeras, wasting wet-lab screening capacity on false hits instead of functional variants.
This whitepaper outlines how an enzymatic assembly workflow using ultra-long oligonucleotides printed directly onto silicon chips removes chimeras from the assembled pool.
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- The impact of chimera formation on screen fidelity and library quality
- A method for generating chimera-free pools of highly similar sequences up to 1.8 kilobases
- Effects of sequence length on synthesis error rates and targeted exploration
