Where is the proteome?

Where a protein is found in a cell can reveal a lot about its function. In the March 15 Genes and Development, Kumar and colleagues report the subcellular localization of 2,744 proteins in the budding yeast, Saccharomyces cerevisiae.

Kumar et al. tagged the proteins with an easy-to-detect epitope, mostly using direct cloning but for some using random transposon mutagenesis, and examined the subcellular localization of the tagged proteins using immunofluorescence. Together with previously published data (some reviewed previously by Pepperkok et al. in Genome Biology, these results mean that the subcellular localization of over half of the yeast proteome is known. The functions of nearly 1,000 of the proteins localized in the study by Kumar et al. were previously unknown, so it may now be possible to explore their potential roles on the basis of where they are found in the cell.

Extrapolating their results to the whole proteome, the ...