Tip Box| Infographic
Best Pipetting Practices
Correct pipetting techniques allow scientists to instantly improve experimental accuracy.
 | Temperature Equilibrium If possible, ensure that the pipette, tips, and liquids are at room temperature. Pre-wet to equilibrate temperature differences and humidity inside the pipette and tip. |  | Optimize Volume Pipette within 35–100 percent of the nominal volume range for air displacement pipettes. When dispensing multiple aliquots, discard the first and last dispense of the series. |
 | Pre-Wet After loading tips, aspirate and dispense the nominal volume 3 times to pre-wet. Without this step, the first few dispenses may deliver less volume. |  | Tackling Tricky Liquids Pipette viscous liquids slowly and volatile liquids quickly. Reverse pipette to avoid volume loss; aspirate the selected volume plus an extra discardable dispense. |
 | How to Pipette Hold the pipette at a consistent angle ≤20 degrees. Immerse the pipette tip 2–3mm below the liquid’s surface and touch off after each dispense. |  | Calibration Calibrate pipettes based on liquid density. When working with nonaqueous liquids, recalibrate the pipette if the liquid has a considerably different density than water. |
| Learn more tips for proper pipetting. | |||
What is your biggest pipetting pain point? | |||
