This webinar will be hosted live and available on-demand

Thursday, April 27, 2023
11:00 AM Eastern Time 

Immunohistochemistry (IHC) has continually evolved alongside technological and scientific advances. Because scientists constantly develop and modify their IHC techniques to better answer modern questions, numerous protocols exist, making IHC potentially confusing and difficult to troubleshoot for newcomers.

In this Technique Talk, Steven Hrycaj will explore the science behind IHC technology and its development over time. He will delve into protocol development, optimization, and troubleshooting, and discuss how sample properties affect protocol decisions.

Learning Objectives

  • IHC background and history
  • Recent IHC advancements
  • Protocol development, optimization, and troubleshooting
  • Manual versus automated protocols using frozen and paraffin-embedded tissue samples
  • Multiplex IHC using colored chromogens


Steven M. Hrycaj, PhD
Assistant Professor
Technical Director, Immunohistochemistry (IHC) Laboratory
Department of Pathology
Michigan Medicine
University of Michigan