Outsourcing your RNA

By Jeffrey M. Perkel Outsourcing your RNA How to get the most molecular bang for your buck when you send your RNA away “The essence of the scientific enterprise,” says Bill Nye, “the Science Guy,” “[is] the Joy of Discovery.”1 But that doesn’t mean it’s practical or even feasible for researchers to do all the discovering themselves. Take RNA expression analysis. With microarrays and next-gen sequencing, in situ

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“The essence of the scientific enterprise,” says Bill Nye, “the Science Guy,” “[is] the Joy of Discovery.”1 But that doesn’t mean it’s practical or even feasible for researchers to do all the discovering themselves.

Take RNA expression analysis. With microarrays and next-gen sequencing, in situ hybridization and qRT-PCR, researchers are answering the most basic of questions, such as what makes a cancer cell a cancer cell.

Yet few investigators have the ability to answer that question entirely on their own. Many, constrained by space, infrastructure, know-how, or time, have no choice but to outsource the work to others. Often, that means employing a university core facility. But some elect to go off campus altogether, enlisting commercial service providers instead.

“The whole application, in terms of both arrays and qRT-PCR, is quite complicated,” says Marie-Louise Lunn, Director of Product Marketing and Communications at Exiqon, a Danish service provider focusing on microRNA ...

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