Scientists working in genetic engineering and pharmaceutical research often find it necessary to obtain highly purified molecules from complex biochemical mixtures. Column chromatography. most often the method of choice, is a time-consuming, labor-intensive, and expensive process. Now, a new method has emerged that is superior to column chromotagraphy in all of these respects. Called radial flow chromotography, this alternative chromatographic method, was recently introduced by Sepragen Corp., San Leandro, Calif. Using this method, scientists will able to obtain purified biomolecules from complex mixtures quickly, more easily, and at a lower cost.
The more common method of aqueous phase column chromatography relies upon three, general pro- cesses: gel filtration, ion exchange, and affinity chromatography. Gel filtration separates molecules on the basis of molecularsize—lower molecular weight molecules are re tained on the porous column matrix, thus eluting from the column after larger molecules. Ion exchange chromatography resolves molecules based on net charge. ...
