Induced pluripotent stem cells (iPSCs) have become very popular in the laboratory for disease modeling, drug discovery, and developmental biology research. However, stem cell expansion and differentiation is a sensitive process, and although numerous strategies have been developed in this sector, there is still ample space to grow. Improving and optimizing stem cell differentiation requires a focus on process knowledge—including finding better strategies for colony selection, media composition, and cell culture; developing new technologies for observing and monitoring cellular behavior at genotypic and phenotypic levels during differentiation; and employing high-throughput screening techniques to assess cell growth, health, and functional properties in a streamlined and automated way.

Download this ebook from Sartorius to learn more about

  • The key factors that drive iPSC differentiation
  • How to build process knowledge through parametric screening
  • Incorporating analytics into experimental design

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